Study of genes involved in the generation of tumor-specific immunity in mice following the injection of H-2Kb-transformed tumor cells

Abstract

We have previously demonstrated that the introduction of allogeneic H-2Kh MHC genes into the AKR leukemic cell line K36 can abrogate the tumorigenicity of the transformed tumor cells. To study the molecular mechanisms involved in mediating the generation of tumor-specific immunity and the genes involved in the process of activation of T cells, one of the transformed clone S38 which expresses high level of the H-2Kf> molecules and can confer strong immunity to (AKR/J X C57BL/6)F1 mice against the parental K36 tumor cells, were used to immunize Fl mice. We had subsequently compared the mRNA isolated from various tissues of the immunized and normal Fl mice by differential display RT-PCR. Several differences in mRNA expression were observed and these differentially expressed sequences were cloned, sequenced and compared to sequences of known genes available in the data bank.