Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/130547
Title: Purification, characterization and substrate specificities of xylanase isoenzymes from Melanocarpus albomyces IIS 68
Authors: Saraswat, V. 
Bisaria, V.S.
Keywords: Characterization
Isoenzyme
Purification
Substrate specificity
Xylanase
Issue Date: Jun-2000
Source: Saraswat, V., Bisaria, V.S. (2000-06). Purification, characterization and substrate specificities of xylanase isoenzymes from Melanocarpus albomyces IIS 68. Bioscience, Biotechnology and Biochemistry 64 (6) : 1173-1180. ScholarBank@NUS Repository.
Abstract: The ascomycetous fungus Melanocarpus albomyces when grown on wheat straw produced seven extracellular xylanase isoenzymes, designated as Ia, Ib, Ic, IIa, IIb, IIc, and IId. All seven xylanases were purified to homogeneity by gel filtration and ion-exchange chromatography. The molecular mass (kDa) of Ia, Ib, Ic, IIa, IIb, IIc, and IId were estimated to be 22.9, 20.7, 18.6, 31.3, 25.4, 38.5, and 34.3, respectively by SDS-PAGE, and 23.7, 20.5, 17.1, 31.7, 25.1, 39.8, and 32.2, respectively by gel filtration. The isoelectric points of Ia, Ib, Ic, IIa and IIb were found to be 6.2, 6.3, 6.4, 3.7, and 4.4, respectively. The activity of the isoenzymes was dependent on the type of the xylan substrates; Xylanases Ia, Ib, and Ic showed highest specific activity toward larchwood xylan (an arabinoglucuronoxylan), IIa and IIc toward birchwood xylan (a glucuronoxylan), and IIb and IId toward beechwood xylan (a glucuronoxylan). Four isoenzymes Ia, Ib, Ic, and IIa had an arabinose-releasing property on larchwood xylan. Application of specific isoenzymes as prebleaching agents in paper manufacture is proposed.
Source Title: Bioscience, Biotechnology and Biochemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/130547
ISSN: 09168451
Appears in Collections:Staff Publications

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