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|Title:||Strategy for the design of custom cDNA microarrays|
|Source:||Lorenz, M.G.O., Cortes, L.M., Lorenz, J.J., Liu, E.T. (2003-06-01). Strategy for the design of custom cDNA microarrays. BioTechniques 34 (6) : 1264-1270. ScholarBank@NUS Repository.|
|Abstract:||DNA microarrays are valuable but expensive tools for expression profiling of cells, tissues, and organs. The design of custom microarrays leads to cost reduction without necessarily compromising their biological value. Here we present a strategy for designing custom cDNA microarrays and constructed a microarray for mouse immunology research (ImmunoChip™). The strategy used interrogates expressed sequence tag databases available in the public domain but overcomes many of the problems encountered. Immunologically relevant clusters were selected based on the expression of expressed sequence tags in relevant libraries. Selected clusters were organized in modules, and the best representative clones were identified. When tested, this microarray was found to have minimal clone identity errors or phage contamination and identified molecular signatures of lymphoid cell lines. Our proposed design of custom microarrays avoids probe redundancy, allows the organization of the chip to optimize chip production, and reduces microarray production costs. The strategy described is also useful for the design of oligonucleotide microarrays.|
|Appears in Collections:||Staff Publications|
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