Please use this identifier to cite or link to this item:
Title: Innate immune responses of pulmonary epithelial cells to Burkholderia pseudomallei infection
Authors: Sim, S.H.
Liu, Y. 
Wang, D.
Novem, V.
Sivalingam, S.P.
Thong, T.W.
Ooi, E.E. 
Tan, G.
Issue Date: 6-Oct-2009
Citation: Sim, S.H., Liu, Y., Wang, D., Novem, V., Sivalingam, S.P., Thong, T.W., Ooi, E.E., Tan, G. (2009-10-06). Innate immune responses of pulmonary epithelial cells to Burkholderia pseudomallei infection. PLoS ONE 4 (10) : -. ScholarBank@NUS Repository.
Abstract: Background: Burkholderia pseudomallei, a facultative intracellular pathogen, causes systemic infection in humans with high mortality especially when infection occurs through an infectious aerosol. Previous studies indicated that the epithelial cells in the lung are an active participant in host immunity. In this study, we aimed to investigate the innate immune responses of lung epithelial cells against B. pseudomallei. Methodology and Principal Findings: Using a murine lung epithelial cell line, primary lung epithelial cells and an inhalational murine infection model, we characterized the types of innate immunity proteins and peptides produced upon B. pseudomallei infection. Among a wide panel of immune components studied, increased levels of major pro-inflammatory cytokines IL-6 and TNFα, chemokine MCP-1, and up-regulation of secretory leukocyte protease inhibitor (SLPI) and chemokine (C-C motif) ligand 20 (CCL20) were observed. Inhibition assays using specific inhibitors suggested that NF-κB and p38 MAPK pathways were responsible for these B. pseudomallei-induced antimicrobial peptides. Conclusions: Our findings indicate that the respiratory epithelial cells, which form the majority of the cells lining the epithelial tract and the lung, have important roles in the innate immune response against B. pseudomallei infection. © 2009 Sim et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0007308
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2009-innate_immune_responses_pulmonary_epithelial-published.pdf390.54 kBAdobe PDF




checked on Jan 16, 2019


checked on Jan 7, 2019

Page view(s)

checked on Nov 9, 2018


checked on Nov 9, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.