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|Title:||Enhanced dehydroepiandrosterone synthesis by amnion compared to chorion: A comparative study using the reverse-isotope dilution technique|
|Authors:||Loganath, A. |
|Source:||Loganath, A., Peh, K.L., Wong, Y.C., Ng, S.C. (2001). Enhanced dehydroepiandrosterone synthesis by amnion compared to chorion: A comparative study using the reverse-isotope dilution technique. Molecular Reproduction and Development 58 (3) : 276-280. ScholarBank@NUS Repository. https://doi.org/10.1002/1098-2795(200103)58:33.0.CO;2-Q|
|Abstract:||With a view to establishing whether the term human fetal membranes possess the enzymic ability to synthesize dehydroepiandrosterone (DHEA) from pregnenolone, homogenates of amnion and chorion obtained from women (n = 5, age 27-34 years) after spontaneous labor at term (37-42 weeks gestation) from uncomplicated pregnancies were incubated with [7n-3H]pregnenolone as substrate. Reverse-isotope dilution analysis gave positive identification of [3H]DHEA acetate in all incubations of viable tissues. No such metabolite was evident in control incubations with heat-denatured tissues. Virtually radiochemically pure esters under three recrystallizations were obtained with mean concentrations of between 15787 and 30137 dpm mol-1 for amnion which was considerably higher than that of chorionic tissues at 4316-5528 dpm mol-1. The magnitude of elevation in DHEA production by amnion was noted to be between 3.6- and 5.5-fold higher than the corresponding chorion. This study provides evidence that the fetal membranes possess 17-α hydroxylase and C-17, 20 lyase activities capable of synthesis of DHEA, an important androgen necessary for aromatization to estrogens in need by the developing fetus. © 2OO1 Wiley-Liss, Inc.|
|Source Title:||Molecular Reproduction and Development|
|Appears in Collections:||Staff Publications|
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