Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/129126
Title: VALIDATION OF MECHANOBIOLOGICAL MARKERS FOR TERATOGEN SCREENING IN DRUG DEVELOPMENT
Authors: CAO YUE
Keywords: mechanobiological markers, teratogen screening, hPSC model, validation, high accuracy, high effiency
Issue Date: 23-May-2016
Citation: CAO YUE (2016-05-23). VALIDATION OF MECHANOBIOLOGICAL MARKERS FOR TERATOGEN SCREENING IN DRUG DEVELOPMENT. ScholarBank@NUS Repository.
Abstract: It has been widely reported that teratogen exposure can lead to various birth defects. Compared with other existing models, the micropatterned human pluripotent stem cell model (μP-hPSC) could not only recapitulate spatially controlled differentiation but also collective cell migration. Preliminary testing results showed that the μP-hPSC model was quite sensitive to five known teratogens. However, without a systematic validation of the model using a larger number of compounds, its application in drug safety would be limited. In this study, 18 compounds were tested using our μP-hPSC model, including 10 known human teratogens and 8 non-teratogens. Validation results here show a competitive accuracy, which is over 94% with only one false positive case (Thiamine) while the highest accuracy of other models is around 91%. Together with high prediction accuracy, operational optimization methods proposed in this study will make μP-hPSC model be a more practical screening application.
URI: http://scholarbank.nus.edu.sg/handle/10635/129126
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