Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/129125
Title: APPLICATIONS OF OLIGOPEPTIDE-FUNCTIONALIZED SURFACES AND MICROPARTICLES IN LIQUID CRYSTALS-BASED ASSAYS
Authors: ONG LIAN HAO
Keywords: liquid crystals, protease assays, oligopeptides, functionalized microparticles, functionalized surfaces, microparticles assembly
Issue Date: 29-Mar-2016
Source: ONG LIAN HAO (2016-03-29). APPLICATIONS OF OLIGOPEPTIDE-FUNCTIONALIZED SURFACES AND MICROPARTICLES IN LIQUID CRYSTALS-BASED ASSAYS. ScholarBank@NUS Repository.
Abstract: Liquid crystals- (LC-) based protease assays are attractive due to its sensitive and multiplexed detection format. However, several key challenges remained. In this study, various strategies were developed to address these issues. Firstly, cysteine-labeled oligopeptides were synthesized for immobilization on solid surfaces, which provide a stable platform for subsequent hydrolysis of oligopeptides by proteases. However, such hydrolysis only causes minute changes to surface topography. Alternatively, bi-cysteine-bearing, biotin-labeled peptides can be used as protease substrates. These peptides have a higher immobilization density and better stability, whereas the biotin moiety offers a streptavidin binding site for amplification of protease activity. Thirdly, microparticles with a high surface area were used as a new platform for protease assays. Surfaces of microparticles were functionalized with octadecyl- (C18)-labeled oligopeptides, which can be cleaved by proteases and influence orientations of LC. Finally, functionalized microparticles assembled at LC/water interfaces are exploited as a functional real-time enzyme sensor.
URI: http://scholarbank.nus.edu.sg/handle/10635/129125
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
OngLH.pdf6.09 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

28
checked on Jan 20, 2018

Download(s)

24
checked on Jan 20, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.