Please use this identifier to cite or link to this item: https://doi.org/10.1002/1529-0131(199808)41:83.0.CO;2-Z
Title: Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis
Authors: Chow, G. 
Nietfeld, J.J.
Knudson, C.B.
Knudson, W.
Issue Date: Aug-1998
Citation: Chow, G.,Nietfeld, J.J.,Knudson, C.B.,Knudson, W. (1998-08). Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis. Arthritis and Rheumatism 41 (8) : 1411-1419. ScholarBank@NUS Repository. https://doi.org/10.1002/1529-0131(199808)41:83.0.CO;2-Z
Abstract: Objective. To better define critical functions of the hyaluronan receptor CD44 in cartilage. Methods. Articular chondrocytes and cartilage tissue slices were treated with CD44 sequence-specific antisense phosphorothioate oligonucleotides. CD44 expression was probed by immunofluorescence microscopy, enzyme-linked immunosorbent assay, and Western blotting. Results. Antisense oligonucleotides demonstrated a dose- and time- dependent inhibition of CD44 protein expression; negative controls showed no effect. Similar to osteoarthritic cartilage, antisense-treated cartilage slices displayed a near-total loss of stainable proteoglycan-rich matrix. Conclusion. CD44 expression is needed for maintenance of cartilage homeostasis.
Source Title: Arthritis and Rheumatism
URI: http://scholarbank.nus.edu.sg/handle/10635/129081
ISSN: 00043591
DOI: 10.1002/1529-0131(199808)41:83.0.CO;2-Z
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

31
checked on Oct 12, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.