Please use this identifier to cite or link to this item:;2-Z
Title: Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis
Authors: Chow, G. 
Nietfeld, J.J.
Knudson, C.B.
Knudson, W.
Issue Date: Aug-1998
Citation: Chow, G.,Nietfeld, J.J.,Knudson, C.B.,Knudson, W. (1998-08). Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis. Arthritis and Rheumatism 41 (8) : 1411-1419. ScholarBank@NUS Repository.;2-Z
Abstract: Objective. To better define critical functions of the hyaluronan receptor CD44 in cartilage. Methods. Articular chondrocytes and cartilage tissue slices were treated with CD44 sequence-specific antisense phosphorothioate oligonucleotides. CD44 expression was probed by immunofluorescence microscopy, enzyme-linked immunosorbent assay, and Western blotting. Results. Antisense oligonucleotides demonstrated a dose- and time- dependent inhibition of CD44 protein expression; negative controls showed no effect. Similar to osteoarthritic cartilage, antisense-treated cartilage slices displayed a near-total loss of stainable proteoglycan-rich matrix. Conclusion. CD44 expression is needed for maintenance of cartilage homeostasis.
Source Title: Arthritis and Rheumatism
ISSN: 00043591
DOI: 10.1002/1529-0131(199808)41:83.0.CO;2-Z
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