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|Title:||Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis|
|Authors:||Chow, G. |
|Citation:||Chow, G.,Nietfeld, J.J.,Knudson, C.B.,Knudson, W. (1998-08). Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis. Arthritis and Rheumatism 41 (8) : 1411-1419. ScholarBank@NUS Repository. https://doi.org/10.1002/1529-0131(199808)41:83.0.CO;2-Z|
|Abstract:||Objective. To better define critical functions of the hyaluronan receptor CD44 in cartilage. Methods. Articular chondrocytes and cartilage tissue slices were treated with CD44 sequence-specific antisense phosphorothioate oligonucleotides. CD44 expression was probed by immunofluorescence microscopy, enzyme-linked immunosorbent assay, and Western blotting. Results. Antisense oligonucleotides demonstrated a dose- and time- dependent inhibition of CD44 protein expression; negative controls showed no effect. Similar to osteoarthritic cartilage, antisense-treated cartilage slices displayed a near-total loss of stainable proteoglycan-rich matrix. Conclusion. CD44 expression is needed for maintenance of cartilage homeostasis.|
|Source Title:||Arthritis and Rheumatism|
|Appears in Collections:||Staff Publications|
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