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|Title:||An enzyme-immobilized microplate for determination of linamarin for large number of samples|
|Authors:||Yeoh, H.-H. |
Catherine Tan, C.-K.
|Citation:||Yeoh, H.-H., Catherine Tan, C.-K. (1994-05). An enzyme-immobilized microplate for determination of linamarin for large number of samples. Biotechnology Techniques 8 (5) : 337-338. ScholarBank@NUS Repository. https://doi.org/10.1007/BF02428977|
|Abstract:||An enzyme-immobilized microplate for determination of linamarin was prepared by covalently linking cassava leaf linamarase to the microplate. For linamarin determination, cassava roots were homogenised in 0.1 M o-phosphoric acid and the filtrate adjusted to pH 6 with NaOH prior to adding into the wells. The cyanide released was then determined spectrophotometrically. One nmol linamarin can be detected. The microplate method is suitable for analysis of large number of samples and is useful for screening purposes. © 1994 Kluwer Academic Publishers.|
|Source Title:||Biotechnology Techniques|
|Appears in Collections:||Staff Publications|
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