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https://doi.org/10.4161/auto.20217
Title: | Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae | Authors: | Deng, Y.Z. Qu, Z. He, Y. Naqvi, N.I. |
Keywords: | Antioxidant Atg20 Fungal virulence GSH Magnaporthe Snx41 Sorting nexin |
Issue Date: | Jul-2012 | Citation: | Deng, Y.Z., Qu, Z., He, Y., Naqvi, N.I. (2012-07). Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae. Autophagy 8 (7) : 1058-1070. ScholarBank@NUS Repository. https://doi.org/10.4161/auto.20217 | Abstract: | The sorting nexins Atg20/Snx42 and Snx41 regulate membrane traffic and endosomal protein sorting and are essential for Cvt and/or pexophagy in yeast. Previously, we showed that macroautophagy is necessary for conidiation in the rice-blast fungus Magnaporthe oryzae. Here, we analyzed the physiological function(s) of selective autophagy in Magnaporthe through targeted deletion of MGG-12832, an ortholog of yeast SNX41 and ATG20/SNX42. Loss of MGG-12832 (hereafter SNX41) abolished conidia formation and pathogenesis in M. oryzae. Snx41-GFP localized as dynamic puncta or short tubules that are partially associated with autophagosomes and/or autophagic vacuoles. PX domain, but not macroautophagy per se, was required for such localization of Snx41-GFP in Magnaporthe. Although not required for nonselective autophagy, Snx41 was essential for pexophagy in Magnaporthe. We identified Oxp1, an ATP-dependent oxoprolinase in the gamma-glutamyl cycle, as a binding partner and potential retrieval target of Snx41-dependent protein sorting. The substrate of Oxp1, 5-oxoproline, could partially restore conidiation in the snx41Δ. Exogenous glutathione, a product of the gamma-glutamyl cycle, significantly restored pathogenicity in the snx41Δ mutant, likely through counteracting the oxidative stress imposed by the host. We propose that the gamma-glutamyl cycle and glutathione biosynthesis are subject to regulation by Snx41-dependent vesicular trafficking, and mediate antioxidant defense crucial for in planta growth and pathogenic differentiation of Magnaporthe at the onset of blast disease in rice. © 2012 Landes Bioscience. | Source Title: | Autophagy | URI: | http://scholarbank.nus.edu.sg/handle/10635/128878 | ISSN: | 15548627 | DOI: | 10.4161/auto.20217 |
Appears in Collections: | Staff Publications |
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