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https://doi.org/10.1038/nmeth.1668
Title: | Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function | Authors: | Zhao, S. Ting, J.T. Atallah, H.E. Qiu, L. Tan, J. Gloss, B. Augustine, G.J. Deisseroth, K. Luo, M. Graybiel, A.M. Feng, G. |
Issue Date: | Sep-2011 | Citation: | Zhao, S., Ting, J.T., Atallah, H.E., Qiu, L., Tan, J., Gloss, B., Augustine, G.J., Deisseroth, K., Luo, M., Graybiel, A.M., Feng, G. (2011-09). Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nature Methods 8 (9) : 745-755. ScholarBank@NUS Repository. https://doi.org/10.1038/nmeth.1668 | Abstract: | Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type-specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type-specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior. © 2011 Nature America, Inc. All rights reserved. | Source Title: | Nature Methods | URI: | http://scholarbank.nus.edu.sg/handle/10635/128698 | ISSN: | 15487091 | DOI: | 10.1038/nmeth.1668 |
Appears in Collections: | Staff Publications |
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