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Title: High-speed optogenetic circuit mapping
Authors: Augustine, G.J. 
Chen, S.
Gill, H.
Katarya, M.
Kim, J.
Kudolo, J.
Lee, L.M.
Lee, H.
Lo, S.Q.
Nakajima, R.
Park, M.-Y.
Tan, G. 
Tang, Y. 
Teo, P. 
Tsuda, S. 
Wen, L.
Yoon, S.-I.
Keywords: brain circuitry
brain imaging
brain mapping
local circuits
Issue Date: 2013
Source: Augustine, G.J., Chen, S., Gill, H., Katarya, M., Kim, J., Kudolo, J., Lee, L.M., Lee, H., Lo, S.Q., Nakajima, R., Park, M.-Y., Tan, G., Tang, Y., Teo, P., Tsuda, S., Wen, L., Yoon, S.-I. (2013). High-speed optogenetic circuit mapping. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 8586 : -. ScholarBank@NUS Repository.
Abstract: Scanning small spots of laser light allows mapping of synaptic circuits in brain slices from transgenic mice expressing channelrhodopsin-2 (ChR2). These light spots photostimulate presynaptic neurons expressing ChR2, while postsynaptic responses can be monitored in neurons that do not express ChR2. Correlating the location of the light spot with the amplitude of the postsynaptic response elicited at that location yields maps of the spatial organization of the synaptic circuits. This approach yields maps within minutes, which is several orders of magnitude faster than can be achieved with conventional paired electrophysiological methods. We have applied this high-speed technique to map local circuits in many brain regions. In cerebral cortex, we observed that maps of excitatory inputs to pyramidal cells were qualitatively different from those measured for interneurons within the same layers of the cortex. In cerebellum, we have used this approach to quantify the convergence of molecular layer interneurons on to Purkinje cells. The number of converging interneurons is reduced by treatment with gap junction blockers, indicating that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affect convergence in sagittal cerebellar slices but not in coronal slices, indicating sagittal polarization of electrical coupling between interneurons. By measuring limb movement or other forms of behavioral output, this approach also can be used in vivo to map brain circuits non-invasively. In summary, ChR2-mediated high-speed mapping promises to revolutionize our understanding of brain circuitry. © 2013 Copyright SPIE.
Source Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ISBN: 9780819493552
ISSN: 16057422
DOI: 10.1117/12.2012404
Appears in Collections:Staff Publications

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