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|Title:||Growing yeast into cylindrical colonies|
Di Meglio, J.-M.
|Citation:||Vulin, C., Di Meglio, J.-M., Lindner, A.B., Daerr, A., Murray, A., Hersen, P. (2014-05-20). Growing yeast into cylindrical colonies. Biophysical Journal 106 (10) : 2214-2221. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bpj.2014.02.040|
|Abstract:||Microorganisms often form complex multicellular assemblies such as biofilms and colonies. Understanding the interplay between assembly expansion, metabolic yield, and nutrient diffusion within a freely growing colony remains a challenge. Most available data on microorganisms are from planktonic cultures, due to the lack of experimental tools to control the growth of multicellular assemblies. Here, we propose a method to constrain the growth of yeast colonies into simple geometric shapes such as cylinders. To this end, we designed a simple, versatile culture system to control the location of nutrient delivery below a growing colony. Under such culture conditions, yeast colonies grow vertically and only at the locations where nutrients are delivered. Colonies increase in height at a steady growth rate that is inversely proportional to the cylinder radius. We show that the vertical growth rate of cylindrical colonies is not defined by the single-cell division rate, but rather by the colony metabolic yield. This contrasts with cells in liquid culture, in which the single-cell division rate is the only parameter that defines the population growth rate. This method also provides a direct, simple method to estimate the metabolic yield of a colony. Our study further demonstrates the importance of the shape of colonies on setting their expansion. We anticipate that our approach will be a starting point for elaborate studies of the population dynamics, evolution, and ecology of microbial colonies in complex landscapes. © 2014 The Authors.|
|Source Title:||Biophysical Journal|
|Appears in Collections:||Staff Publications|
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