PROTEOMICS AND METABOLOMICS INVESTIGATION OF GREEN ALGAE UPON EXPOSURE TO PESTICIDES

Abstract

The development of proteomics and metabolomics approaches for evaluation of pesticides exposure to green algae was explored in this dissertation. The evaluation of different protein extraction methods using a combination of shotgun proteomics and SWATH approach demonstrated that phenol-TCA/acetone method was the most effective. The determination of cypermethrin effect on algae proteome regarding its exposure concentrations and durations was performed using iTRAQ approach. The biological interpretation of these altered proteins indicated that cypermethrin could affect biological processes in algae including photosynthesis, stress response and carbohydrate metabolism. The metabolic response of algae to various diazinon concentrations for different durations was investigated using LC-MS based metabolomics approach. Diazinon mainly affects metabolites like fatty acids, carbohydrates, glycerolipids and glycerophospholipids and biological pathways including lipid metabolism, glycosylphosphatidylinositol-anchor metabolism and inositol phosphate metabolism in green algae.