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|Title:||Expression of the Chlamydomonas reinhardtii Sedoheptulose-1,7-bisphosphatase in Dunaliella bardawil leads to enhanced photosynthesis and increased glycerol production|
|Authors:||Fang, L. |
|Citation:||Fang, L., Lin, H.X., Low, C.S., Wu, M.H., Chow, Y., Lee, Y.K. (2012-12). Expression of the Chlamydomonas reinhardtii Sedoheptulose-1,7-bisphosphatase in Dunaliella bardawil leads to enhanced photosynthesis and increased glycerol production. Plant Biotechnology Journal 10 (9) : 1129-1135. ScholarBank@NUS Repository. https://doi.org/10.1111/pbi.12000|
|Abstract:||Bioengineering of photoautotrophic microalgae into CO2 scrubbers and producers of value-added metabolites is an appealing approach in low-carbon economy. A strategy for microalgal bioengineering is to enhance the photosynthetic carbon assimilation through genetically modifying the photosynthetic pathways. The halotolerant microalgae Dunaliella posses an unique osmoregulatory mechanism, which accumulates intracellular glycerol in response to extracellular hyperosmotic stresses. In our study, the Calvin cycle enzyme sedoheptulose 1,7-bisphosphatase from Chlamydomonas reinhardtii (CrSBPase) was transformed into Dunaliella bardawil, and the transformant CrSBP showed improved photosynthetic performance along with increased total organic carbon content and the osmoticum glycerol production. The results demonstrate that the potential of photosynthetic microalgae as CO2 removers could be enhanced through modifying the photosynthetic carbon reduction cycle, with glycerol as the carbon sink. © 2012 The Authors Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.|
|Source Title:||Plant Biotechnology Journal|
|Appears in Collections:||Staff Publications|
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