Please use this identifier to cite or link to this item:
|Title:||Ion transport function of SLC4A11 in corneal endothelium|
|Citation:||Jalimarada, S.S., Ogando, D.G., Vithana, E.N., Bonanno, J.A. (2013). Ion transport function of SLC4A11 in corneal endothelium. Investigative Ophthalmology and Visual Science 54 (6) : 4330-4340. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.13-11929|
|Abstract:||Purpose. Mutations in SLC4A11, a member of the SLC4 superfamily of bicarbonate transporters, give rise to corneal endothelial cell dystrophies. SLC4A11 is a putative Na+ borate and Na+:OH- transporter. Therefore we ask whether SLC4A11 in corneal endothelium transports borate (B[OH]4 -), bicarbonate (HCO3 -), or hydroxyl (OH-) anions coupled to Na++. Methods. SLC4A11 expression in cultured primary bovine corneal endothelial cells (BCECs) was determined by semiquantitative PCR, SDS-PAGE/Western blotting, and immunofluorescence staining. Ion transport function was examined by measuring intracellular pH (pHi) or Na+ ([Na+]i) in response to Ringer solutions with/without B(OH)4 - or HCO3 - after overexpressing or small interfering RNA (siRNA) silencing of SLC4A11. Results. SLC4A11 is localized to the basolateral membrane in BCEC. B(OH)4 - (2.5-10 mM) in bicarbonate-free Ringer induced a rapid small acidification (0.01 pH unit) followed by alkalinization (0.05-0.1 pH unit), consistent with diffusion of boric acid into the cell followed by B(OH)4 -. However, the rate of B(OH)4 --induced pHi change was unaffected by overexpression of SLC4A11. B(OH)4 - did not induce significant changes in resting [Na+ i] or the amplitude and rate of acidification caused by Na+ removal. siRNA-mediated knockdown of SLC4A11 (~70%) did not alter pHi responses to CO2/HCO3 --rich Ringer, Na+-free induced acidification, or the rate of Na+ influx in the presence of bicarbonate. However, in the absence of bicarbonate, siSLC4A11 knockdown significantly decreased the rate (43%) and amplitude (48%) of acidification due to Na+ removal and recovery (53%) upon add-back. Additionally, the rate of acid recovery following NH4+ prepulse was decreased significantly (27%) by SLC4A11 silencing. Conclusions. In corneal endothelium, SLC4A11 displays robust Na+-coupled OH- transport, but does not transport B(OH)4- or HCO3-. © 2013 The Association for Research in Vision and Ophthalmology, Inc.|
|Source Title:||Investigative Ophthalmology and Visual Science|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 9, 2018
WEB OF SCIENCETM
checked on Oct 9, 2018
checked on Sep 21, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.