Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.antiviral.2012.05.017
Title: Induction of protective immune responses against EV71 in mice by baculovirus encoding a novel expression cassette for capsid protein VP1
Authors: Premanand, B.
Kiener, T.K.
Meng, T.
Tan, Y.R.
Jia, Q.
Chow, V.T.K.
Kwang, J. 
Keywords: Baculovirus surface display
Enterovirus 71 (EV71)
HA transmembrane anchor
Immune responses
VP1
Issue Date: Sep-2012
Citation: Premanand, B., Kiener, T.K., Meng, T., Tan, Y.R., Jia, Q., Chow, V.T.K., Kwang, J. (2012-09). Induction of protective immune responses against EV71 in mice by baculovirus encoding a novel expression cassette for capsid protein VP1. Antiviral Research 95 (3) : 311-315. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2012.05.017
Abstract: EV71 is a major causative agent of hand, foot and mouth disease (HFMD) and is responsible for large outbreaks in various Asian Pacific countries. In the present study, we generated the recombinant baculovirus (Bac-VP1) encoding VP1 in a novel expression cassette. The transmembrane domain of hemagglutinin of the H3N2 influenza virus was included in the cassette as a minimal membrane anchor for VP1. The protective immunity of Bac-VP1 was investigated in a mouse model. The results showed that mice vaccinated with live Bac-VP1 had strong VP1 specific antibody responses. In an in vitro neutralization assay Bac-VP1 sera exhibited cross-neutralization against homologous and heterologous EV71 strains with a maximum titer of 1:512. Passive immunization studies confirmed that these sera were able to provide 100% protection against 5 MLD50 of mouse adapted EV71 (B4 strain). This study revealed that baculovirus displaying VP1 with a HA transmembrane domain efficiently induced cross-neutralizing antibody responses in mice. © 2012 Elsevier B.V.
Source Title: Antiviral Research
URI: http://scholarbank.nus.edu.sg/handle/10635/125503
ISSN: 01663542
DOI: 10.1016/j.antiviral.2012.05.017
Appears in Collections:Staff Publications

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