Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/125164
Title: METHODOLOGY DEVELOPMENT FOR ENVIRONMENTAL MICROBIOLOGY: MEDIUM FORMULATION AND SURFACE CHARGE ANALYSIS
Authors: NG WENFA
Keywords: Medium formulation, Colourless agar, As-yet-not-cultivated problem, Surface charge analysis
Issue Date: 3-Apr-2012
Citation: NG WENFA (2012-04-03). METHODOLOGY DEVELOPMENT FOR ENVIRONMENTAL MICROBIOLOGY: MEDIUM FORMULATION AND SURFACE CHARGE ANALYSIS. ScholarBank@NUS Repository.
Abstract: Analytical techniques afford scientists the tools to see further and deeper into their subjects of investigation. This thesis attempted to develop new and improved methods in the areas of cultivation of microorganisms from environmental water samples and bacterial surface charge analysis. Firstly, a colourless agar medium was formulated in an attempt to solve the ?Great Plate Count Anomaly? problem. Secondly, as there is no consensus on the type of wash buffer for the preparation of bacterial biomass for surface charge analysis, a systematic evaluation of the effectiveness of various wash buffers in removing nonspecifically adsorbed ions and charged molecules from the bacterial cell surface was performed.
URI: http://scholarbank.nus.edu.sg/handle/10635/125164
Appears in Collections:Master's Theses (Open)

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