QUANTITATIVE ANALYSIS OF THE MOVEMENT OF AGROBACTERIUM-DELIVERED VIRE2 PROTEINS INSIDE PLANT CELL

Abstract

AGROBACTERIUM TUMEFACIENS IS A NATURAL GENETIC ENGINEER WIDELY USED TO DELIVER DNA AND VIRE2 INTO PLANT CELLS. A RECENTLY DEVELOPED SPLIT-GFP APPROACH HAS BEEN PROVED TO BE ONE OF THE CONSIDERABLE METHODS TO DIRECTLY VISUALIZE THE MOVEMENT OF AGROBACTERIUM-DELIVERED VIRE2 PROTEIN (MARKED WITH GFP) INSIDE PLANT CELL. IN THIS REPORT, THE QUANTITATIVE CHARACTERISTICS OF THE MOVEMENT OF AGROBACTERIUM-DELIVERED VIRE2 PROTEIN AND THE EFFECTS OF THREE KINDS OF PROTEIN TRANSFERRING INHIBITORS(BDM, BFA AND CYTOD) ON VIRE2 MOVEMENT WERE ANALYZED INSIDE PLANT CELL. PREVIOUS WORK WITH THE SPLIT-GFP APPROACH HAD BEEN DONE IN MY LAB TO GET CONFOCAL TIME-LAPSE IMAGES OF AGROBACTERIUM-DELIVERED VIRE2 MOVEMENT IN PLANT RECIPIENT CELLS, AS WELL AS IN PROTEIN TRANSFER INHIBITED CELLS. HAVING TRACKED MOVING VIRE2 AND QUANTITATIVE ANALYZED VIRE2 MOVING TRACK IN THE TIME-LAPSE CONFOCAL IMAGES, VIRE2 WERE FOUND TO BE DIRECTIVE DELIVERED FROM CELLAR CYTOPLASM INTO NUCLEUS WITH AVERAGE MOVING SPEED OF 0.62 UM/