Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/123714
Title: EZH2 AND FOXM1 CROSSTALK IN TRIPLE NEGATIVE BREAST CANCER
Authors: SYLVIA MAHARA
Keywords: EZH2, FOXM1, MMP, TNBC, Proliferation, Invasion
Issue Date: 13-Aug-2015
Citation: SYLVIA MAHARA (2015-08-13). EZH2 AND FOXM1 CROSSTALK IN TRIPLE NEGATIVE BREAST CANCER. ScholarBank@NUS Repository.
Abstract: Both EZH2 and FOXM1 have been consistently shown to be upregulated in aggressive breast cancer and in this study, we sought to dissect the crosstalk between EZH2 and FOXM1 in TNBC. By combining microarray data in TNBC cell line with TCGA patient database, we observed a positive correlation between EZH2 and FOXM1. Subsequent in-vitro cell-based assays showed that EZH2 positively modulated FOXM1-mediated invasion pathway with co-occupancy of EZH2 and FOXM1 on the promoter of MMP2 and MMP7. Interestingly, the canonical function of EZH2 was not completely loss. In accordance to this, we also identified occupancy of PRC2 complex and its associated repressive H3K27me3 mark on the same MMPs promoters. Taken together, this study proposed a model highlighting dual regulation of EZH2 in regulating MMP-mediated invasion in TNBC subtype. The balance between canonical and non-canonical roles of EZH2 could possibly be the determining factor in promoting a more aggressive breast cancer.
URI: http://scholarbank.nus.edu.sg/handle/10635/123714
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
MaharaS.pdf3.47 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

117
checked on Dec 14, 2018

Download(s)

135
checked on Dec 14, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.