Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/122947
Title: LINE SCAN FOCAL MODULATION MICROSCOPY FOR RAPID IMAGING OF THICK BIOLOGICAL SAMPLES
Authors: PANT SHILPA
Keywords: Optical, Microscope, Line-scan, Deep Imaging, High-Speed
Issue Date: 6-Aug-2015
Citation: PANT SHILPA (2015-08-06). LINE SCAN FOCAL MODULATION MICROSCOPY FOR RAPID IMAGING OF THICK BIOLOGICAL SAMPLES. ScholarBank@NUS Repository.
Abstract: LINE-SCAN FOCAL MODULATION MICROSCOPY IS A HIGH-SPEED, HIGH-RESOLUTION IMAGING TECHNIQUE CAPABLE OF DEEP IMAGING OF THICK BIOLOGICAL TISSUES. THIS THESIS DESCRIBES THREE DIFFERENT IMPLEMENTATIONS OF THE LINE-SCANNING FOCAL MODULATION MICROSCOPE. THE PERFORMANCE OF THE MICROSCOPE IS VALIDATED BY IMAGING FLUORESCENT BEADS EMBEDDED IN A SCATTERING GEL. THE SIGNAL-TO-BACKGROUND RATIO FOR THE LINE-SCAN FMM SYSTEM IS ABOUT 8 TIMES HIGHER THAN FOR LINE-SCANNING CONFOCAL SYSTEMS EVEN AT DEPTHS >100 ?M. THUS, THIS SYSTEM RETAINS ITS SECTIONING CAPABILITY EVEN AT GREAT DEPTHS. THE LINE-SCAN FMM SYSTEM HAS BEEN USED TO IMAGE THE BLOOD FLOW IN VARIOUS ZEBRAFISH MODELS. COMPARISON OF THE LINE-SCAN FMM IMAGE WITH DIFFERENT COMMERCIAL MICROSCOPES SHOWS THAT THE FMM SYSTEM HAS BETTER SPATIAL AND TEMPORAL RESOLUTION AND IS WELL SUITED FOR HIGH-SPEED IN VIVO IMAGING IN BIOLOGICAL TISSUES.
URI: http://scholarbank.nus.edu.sg/handle/10635/122947
Appears in Collections:Ph.D Theses (Open)

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