Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/122316
Title: TRANSCRIPTIONAL REPRESSION MECHANISM OF TROCARIN D, A SNAKE VENOM PROTHROMBIN ACTIVATOR FROM TROPIDECHIS CARINATUS
Authors: HAN XIA
Keywords: Transcription regulation of venom toxins, toxin gene evolution, AG-rich sequences, silencing cis-element, polycomb response element, YY1
Issue Date: 23-Jan-2015
Citation: HAN XIA (2015-01-23). TRANSCRIPTIONAL REPRESSION MECHANISM OF TROCARIN D, A SNAKE VENOM PROTHROMBIN ACTIVATOR FROM TROPIDECHIS CARINATUS. ScholarBank@NUS Repository.
Abstract: Trocarin D (TroD), a venom prothrombin activator from Tropidechis carinatus shares similar structure and function with blood coagulation factor Xa (TrFXa). Their distinct physiological roles are due to distinct expression patterns. The genes of TroD and TrFX are highly similar, except for promoter and intron 1. The promoter insertion in TroD accounts for the elevated but not venom gland-specific expression. We examined the roles of three insertions and two deletions in intron 1 of TroD in the regulation of expression, and found a 209-bp region within the second insertion silences expression. We identified five AG-rich motifs in this region. All except the 5th motif are important for silencing function via recruitment of YY1, Sp3 and HMGB2. Similar AG-rich motif clusters are found in other toxin genes but not in their physiological counterparts. Thus, AG-rich motifs contribute to regulation of expression of TroD, and probably other toxin genes.
URI: http://scholarbank.nus.edu.sg/handle/10635/122316
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