Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121777
Title: INSIGHTS INTO CD8 T CELL-MEDIATED PATHOLOGY DURING EXPERIMENTAL CEREBRAL MALARIA
Authors: POH CHEK MENG
Keywords: Malaria, ECM, CD8 T cells, Immunology, MHC class I, Cross-presentation
Issue Date: 30-Jul-2015
Citation: POH CHEK MENG (2015-07-30). INSIGHTS INTO CD8 T CELL-MEDIATED PATHOLOGY DURING EXPERIMENTAL CEREBRAL MALARIA. ScholarBank@NUS Repository.
Abstract: Cerebral malaria is a fulminant complication in severe malaria infections that is responsible for the wide majority of deaths in malaria. In the mouse model, CD8 T cells play a pathogenic role in experimental cerebral malaria (ECM), but the epitopes they recognize remains unknown. In my PhD project, a PbA cDNA library enriched in open reading frames was constructed, expressed in EL4 cells and screened against T reporter cell lines bearing TCRs from CD8 T cells sequestered in the brains of mice during ECM. Two novel epitopes were discovered this way. Functional malaria-specific CD8 T cells accumulate in the brains, where cross-presentation of cognate epitopes occur in the cells lining blood-brain barrier (BBB) during ECM. Further experiments indicate that CD8 T cells of multiple malaria specificities synergistically damage BBB in the brains, suggesting inhibition of cross-presentation might have therapeutic potential in cerebral malaria.
URI: http://scholarbank.nus.edu.sg/handle/10635/121777
Appears in Collections:Ph.D Theses (Open)

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