EFFECT OF NATURAL AND SYNTHETIC COLLAGEN CROSS-LINKING AGENTS ON THE BOND STRENGTH AND DURABILITY OF RESIN-DENTIN INTERFACE

Abstract

AIMS: TO INVESTIGATE THE EFFECT OF A SYNTHETIC [CARBODIIMIDE (EDC)] AND A NATURAL [GRAPE SEED EXTRACT (GSE)] COLLAGEN CROSS-LINKING AGENT ON THE BOND STRENGTH AND DURABILITY OF THE RESIN-DENTIN INTERFACE USING ETCH-AND-RINSE (E&R) ADHESIVE SYSTEM AND A NEWLY INTRODUCED UNIVERSAL ADHESIVE (UA) SYSTEM. ADDITIONALLY THE EFFECT OF DRY AND WET BONDING ON THE RESIN-DENTIN INTERFACE CREATED BY UA WAS EVALUATED. METHODS: THE STUDY WAS DIVIDED INTO TWO PHASES, THE FIRST INVOLVING EDC, UA AND A THREE-STEP E&R ADHESIVE SYSTEM AND THE SECOND PHASE INVOLVING GSE, UA AND A TWO-STEP E&R ADHESIVE SYSTEM. SCANNING ELECTRON MICROSCOPY ANALYSIS, MICROTENSILE BOND STRENGTH TESTING AND NANOLEAKAGE EVALUATION WERE THE INVESTIGATIONS DONE. RESULTS: THE USE OF EDC AS A PRE-CONDITIONER HELPS STABILIZE THE INTERFACE CREATED BY THE 3-STEP E&R AND UA ESPECIALLY IN DRY BONDING CONDITIONS WHEREAS GSE AS A PRE-TREATMENT DID NOT SIGNIFICANTLY IMPROVE THE BOND STRENGTH AND DURABILITY OF THE TESTED RESIN-DENTIN INTER