Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121737
Title: THE MECHANISM OF JUNB TRANSCRIPTIONAL REGULATION UNDER TLR ACTIVATION IN MOUSE MACROPHAGES
Authors: LU NING
Keywords: JunB, macrophages, TLRs, crosstalk, transcription factors, innate immunity
Issue Date: 17-Aug-2015
Source: LU NING (2015-08-17). THE MECHANISM OF JUNB TRANSCRIPTIONAL REGULATION UNDER TLR ACTIVATION IN MOUSE MACROPHAGES. ScholarBank@NUS Repository.
Abstract: Toll-like receptor (TLR) - pathway crosstalk caused by multiple PAMPs induces proinflammatory cytokine synergy in macrophages. Several potential synergistic factors including JunB have been found. But the mechanisms of junb regulation in macrophages are unclear. In this thesis work, we showed that junb mRNA and JunB protein were induced under single and combinatorial PAMP stimulations in macrophages. A junb downstream enhancer region significantly up-regulates junb transcription when cooperating with junb promoter. Mutagenesis analysis showed that four NF-?B sites in junb enhancer region are important for junb transcription. Furthermore, the junb promoter and enhancer co-pulldown assay followed by mass spectrometry analysis facilitated our identification of potential transcription factors including AATF, SSRP1 and TARDBP, which may regulate junb under different PAMP stimulations. Our findings delineate the functional regions in regulating junb and provide potential factors that may regulate junb under different PAMP stimulations.
URI: http://scholarbank.nus.edu.sg/handle/10635/121737
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
LuN.pdf2.19 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

64
checked on Feb 16, 2018

Download(s)

43
checked on Feb 16, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.