Please use this identifier to cite or link to this item: https://doi.org/10.1007/s004410051183
Title: Ontogeny of NADPH-d expression in the thymic microenvironment of the chick embryo
Authors: Gulati, P. 
Leong, S.-K. 
Chan, A.-S. 
Keywords: Chicken
Embryo
Nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADPH-d)
Nitric oxide
Thymus
Issue Date: 1998
Citation: Gulati, P., Leong, S.-K., Chan, A.-S. (1998). Ontogeny of NADPH-d expression in the thymic microenvironment of the chick embryo. Cell and Tissue Research 294 (2) : 335-343. ScholarBank@NUS Repository. https://doi.org/10.1007/s004410051183
Abstract: Nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry was used to demonstrate the presence of nitric oxide in the developing chicken thymus. NADPH-d was first expressed in the epithelial cells located at the corticomedullary junction of the thymic rudiment on day 13 of incubation. The number of labelled cells gradually increased from day 13 to day 21. Ultrastructural evidence showed that the labelling was localized in a heterogeneous population of cells in the medulla near the corticomedullary junction, comprising the cystic, undifferentiated, myoid, lymphoid and epithelial reticular cells. At this age, the vascular endothelium was NADPH-d positive. Labelling was also detected in some macrophages. The reaction product primarily labelled profiles of rough endoplasmic reticulum and to a lesser extent the outer membranes of mitochondria, portions of the nuclear envelope and the Golgi apparatus. By day 18/19, NADPH-d-labelled nerve fibres were occasionally observed in the interlobular connective tissue. By day 21, these fibres formed perivascular plexuses. Labelled nerve fibres were occasionally observed in the medullary parenchyma. Possible functions of nitric oxide in the embryonic thymus are discussed.
Source Title: Cell and Tissue Research
URI: http://scholarbank.nus.edu.sg/handle/10635/120774
ISSN: 0302766X
DOI: 10.1007/s004410051183
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