A STUDY OF SAMD9 AND PSMB9 AS POTENTIAL DOWNSTREAM MOLECULES OF HS6ST3 IN HUMAN BREAST CANCER

Abstract

BREAST CANCER IS THE MOST COMMONLY DIAGNOSED CANCER AMONG FEMALE. DESPITE ADVANCES IN CANCER TREATMENT, BREAST CANCER HAS CONTRIBUTED TO MOST OF FEMALE CANCER DEATH NOWADAYS. HENCE SEARCHING FOR BIOMARKER AND THERAPEUTIC TARGET REMAINED AS TOP PRIORITY IN BREAST CANCER RESEARCH. HEPARAN SULFATE 6-O SULFOTRANSFERASE 3 (HS6ST3) IS A POTENTIAL TARGET IN THIS REGARD. HS6ST3 IS A KEY REGULATOR OF BREAST CANCER PHENOTYPES. SILENCING OF HS6ST3 WAS FOUND TO SUPPRESS BREAST CANCER GROWTH, INVASION AND MIGRATION WHILE INDUCING APOPTOSIS. UNDERSTANDING OF UNDERLYING MECHANISM THAT LEAD TO PHENOTYPIC CHANGES WOULD BE USEFUL TO EXPLORE ALTERNATE ROUTE THAT CONTRIBUTE TO BREAST CANCER PROGRESSION. THE AIM OF THIS STUDY WAS TO IDENTIFY POTENTIAL DOWNSTREAM MOLECULES OF HS6ST3 IN HUMAN BREAST CANCER. DNA MICROARRAY WAS USED IN HS6ST3-SILENCED MCF7 BREAST CANCER CELL LINE TO ASSESS GENOME WIDE EXPRESSION CHANGES. ANALYZED DATA SHOWED THAT THERE WERE 220 UPREGULATED GENES AND 159 DOWNREGULATED GENES WIT