Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/118616
Title: BIOCHEMICAL CHARACTERIZATION OF ETHANOL MODIFIED PHOSPHOLIPIDS IN SACCHAROMYCES CEREVISIAE
Authors: SHEN TONG
Keywords: Phosphatidylethanol, ethanol, S. cerevisiae, liquid chromatography, mass spectrometry, lipidomics
Issue Date: 14-Aug-2014
Source: SHEN TONG (2014-08-14). BIOCHEMICAL CHARACTERIZATION OF ETHANOL MODIFIED PHOSPHOLIPIDS IN SACCHAROMYCES CEREVISIAE. ScholarBank@NUS Repository.
Abstract: PHOSPHATIDYLETHANOL (PETH) IS REGARDED AS AN ABNORMAL LIPID IN CELLULAR MEMBRANE, UNIQUELY FORMED FROM PHOSPHATIDYLCHOLINE VIA PHOSPHOLIPASE D-CATALYZED TRANSPHOSPHATIDYLATION IN THE PRESENCE OF ETHANOL. IT HAS A DISTINCTLY DIFFERENT CHEMICAL STRUCTURE COMPARED WITH OTHER PHOSPHOLIPIDS AND MAY PERTURB THE PHYSICOCHEMICAL PROPERTIES OF CELL MEMBRANES. PETH HAS BEEN SHOWN TO BE INVOLVED IN ETHANOL TOLERANCE IN CELLULAR MEMBRANES IN VITRO, WHICH INDICATES A POSSIBLE ROLE FOR PETH IN YEAST ADAPTATION TO SELF-PRODUCED ETHANOL DURING FERMENTATION. HOWEVER, ENDOGENOUSLY FORMED PETH IN SACCHAROMYCES CEREVISIAE HAS NEVER BEEN ELUCIDATED. OUR STUDY AIMED TO PAVE THE WAY TO ILLUSTRATE A FULL PROFILE FOR PETH IN FERMENTING YEAST AND ITS MODULATORY ROLE IN MEMBRANE DYNAMICS DURING FERMENTATION. LIPIDOMICS STRATEGIES, DRIVEN BY MODERN MASS SPECTROMETRY TECHNIQUES, ARE ABLE TO PROVIDE POWERFUL SENSITIVITY AND SELECTIVITY TO CHARACTERIZE SUCH A PHYSIOLOGICALLY RARE PHOSPHOLIPID.
URI: http://scholarbank.nus.edu.sg/handle/10635/118616
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
SHENTong_PhD_2014.pdf4.88 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

145
checked on Jan 19, 2018

Download(s)

20
checked on Jan 19, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.