Please use this identifier to cite or link to this item:
|Title:||Serine 312 phosphorylation is dispensable for wild-type p53 functions in vivo|
|Citation:||Lee, M.K., Tong, W.M., Wang, Z.Q., Sabapathy, K. (2011-02). Serine 312 phosphorylation is dispensable for wild-type p53 functions in vivo. Cell Death and Differentiation 18 (2) : 214-221. ScholarBank@NUS Repository. https://doi.org/10.1038/cdd.2010.90|
|Abstract:||Cellular stimulation results in phosphorylation of the tumor suppressor p53 on multiple residues, though the functional relevance is not always clear. It is noteworthy that the serine (S) 315 residue is unique, as it has been suggested to be phosphorylated not only by genotoxic signals, but also during cell-cycle progression and by endoplasmic-reticulum stress. However, in vitro data have been conflicting as phosphorylation at this site was shown to both positively and negatively regulate p53 functions. We have thus generated knock-in mice expressing an unphosphorylable S312 (equivalent to human S315), by substitution with an alanine (A) residue, to clarify the conflicting observations and to evaluate its functional relevance in vivo. Born at Mendelian ratios, the p53 S312A/S312Amice show no anomalies during development and adulthood. p53 activation, stability, localization and ability to induce apoptosis, cell-cycle arrest and prevent centrosome amplification are not compromised in p53 S312A/S312Acells. p53 S312A/S312A mice are unable to rescue mdm2 / lethality, and tumorigenesis- both spontaneous and irradiation/oncogene-induced- is not accentuated. Taken together, the results show that the S312 phosphorylation site is not in itself necessary for efficient p53 function, and advocates the possibility that it is neither relevant in the mouse context nor important for p53 functions in vivo. © 2011 Macmillan Publishers Limited All rights reserved.|
|Source Title:||Cell Death and Differentiation|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 19, 2018
WEB OF SCIENCETM
checked on Sep 5, 2018
checked on Jul 27, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.