Please use this identifier to cite or link to this item:
Title: Role of prefrontal cortical calcium independent phospholipase A2 in antidepressant-like effect of maprotiline
Authors: Lee, L.H.-W.
Tan, C.-H.
Shui, G. 
Wenk, M.R.
Ong, W.-Y.
Keywords: Antidepressants
calcium-independent phospholipase A2
docosahexaenoic acid
prefrontal cortex
Issue Date: Sep-2012
Citation: Lee, L.H.-W., Tan, C.-H., Shui, G., Wenk, M.R., Ong, W.-Y. (2012-09). Role of prefrontal cortical calcium independent phospholipase A2 in antidepressant-like effect of maprotiline. International Journal of Neuropsychopharmacology 15 (8) : 1087-1098. ScholarBank@NUS Repository.
Abstract: There is increasing interest in the pathophysiology and neurochemistry of the prefrontal cortex (PFC) in depression. Blood flow and metabolism are decreased in the PFC of patients with depression compared to controls. Changes in long-chain polyunsaturated fatty acids (PUFAs) are also associated with depression. This study was conducted to elucidate a possible role of PFC activity of an enzyme involved in the release of docosahexaenoic acid (DHA), i.e. calcium-independent phospholipase A2 (iPLA2), in the effects of the norepinephrine reuptake inhibitor (NRI) antidepressant, maprotiline, in mice. Treatment of Balb/C mice with maprotiline for 4 wk resulted in reduction in the level of behavioural despair, as determined by decreased immobility and increased climbing during the forced swim test. In contrast, mice treated with maprotiline plus bilateral prefrontal cortical injections of antisense oligonucleotide to iPLA2, showed significantly increased immobility and decreased climbing, to levels comparable to saline-treated controls, indicating abolishment of the antidepressant-like effect of maprotiline. Lipidomic analyses showed significant decreases in phosphatidylcholine species containing long-chain PUFAs and increases in lysophosphatidylcholine after maprotiline treatment, indicating increased PLA2 activity and endogenous release of eicosapentaenoic acid (EPA) or DHA after maprotiline treatment. These changes in lipid profiles were absent in mice that received maprotiline and PFC injections of antisense oligonucleotide to iPLA2. Together, the results indicate that PFC iPLA2 activity plays an important role in the antidepressant-like effect of maprotiline, possibly through endogenous release of long-chain PUFAs. © 2011 CINP.
Source Title: International Journal of Neuropsychopharmacology
ISSN: 14611457
DOI: 10.1017/S1461145711001234
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Dec 13, 2018


checked on Dec 13, 2018

Page view(s)

checked on Nov 23, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.