Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-61779-319-6_13
Title: Nanostructured TiO 2 thin films for phosphoproteomics studies with MALDI mass spectrometry
Authors: Torta, F. 
Fusi, M.
Casari, C.S.
Bassi, A.L.
Bachi, A.
Keywords: MALDI
Mass spectrometry
Phosphoproteomics
Pulsed laser deposition
Titanium dioxide
Issue Date: 2011
Citation: Torta, F.,Fusi, M.,Casari, C.S.,Bassi, A.L.,Bachi, A. (2011). Nanostructured TiO 2 thin films for phosphoproteomics studies with MALDI mass spectrometry. Methods in Molecular Biology 790 : 173-181. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-61779-319-6_13
Abstract: Alterations in protein phosphorylation, a posttranslational modification (PTM) that regulates many-processes in living cells, is a fundamental mechanism of many diseases, including cancer. Phosphoproteomics, with the combined use of affinity chromatography and electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, is shedding light into phosphorylation signaling pathways at the proteome level and helps to solve difficulties related to sample complexity and phosphopeptide enrichment. One of the most frequent and efficient methods used to enrich samples for the phosphorylated components is titanium dioxide chromatography. Titanium dioxide has a high affinity for phosphopeptides and can also be selective in specific experimental conditions. Here, we describe a protocol for the use of a MALDI plate covered with titanium dioxide nanostructured film, a device developed for a rapid and efficient study of phosphorylated peptides. © 2011 Springer Science+Business Media, LLC.
Source Title: Methods in Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/117087
ISBN: 9781617793189
ISSN: 10643745
DOI: 10.1007/978-1-61779-319-6_13
Appears in Collections:Staff Publications

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