Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2005.11.103
Title: Simultaneous determination of synthetic phosphodiesterase-5 inhibitors found in a dietary supplement and pre-mixed bulk powders for dietary supplements using high-performance liquid chromatography with diode array detection and liquid chromatography-electrospray ionization tandem mass spectrometry
Authors: Zou, P.
Oh, S.S.-Y.
Hou, P.
Low, M.-Y.
Koh, H.-L. 
Keywords: Acetildenafil
Adulterants
Dietary supplement
Homosildenafil
Hydroxyhomosildenafil
LC-ESI-MS/MS
PDE-5 inhibitor
Sildenafil
Tadalafil
Vardenafil
Issue Date: 3-Feb-2006
Citation: Zou, P., Oh, S.S.-Y., Hou, P., Low, M.-Y., Koh, H.-L. (2006-02-03). Simultaneous determination of synthetic phosphodiesterase-5 inhibitors found in a dietary supplement and pre-mixed bulk powders for dietary supplements using high-performance liquid chromatography with diode array detection and liquid chromatography-electrospray ionization tandem mass spectrometry. Journal of Chromatography A 1104 (1-2) : 113-122. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2005.11.103
Abstract: A high-performance liquid chromatography-diode array detection (HPLC-DAD) method and a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method were developed to screen for the presence of synthetic phosphodiesterase-5 (PDE-5) inhibitors and their analogues, namely sildenafil, vardenafil, tadalafil, homosildenafil, acetildenafil and hydroxyhomosildenafil. The methods were applied to pre-market samples submitted to the Health Sciences Authority of Singapore (HSA) for testing. One sample was in the form of capsules while six other samples were pre-mixed bulk powder samples for dietary supplements to be repackaged or formulated into the final dosage forms (usually capsules). Identification of PDE-5 inhibitors and their analogues was achieved by comparing individual peak retention times, UV spectra and mass spectra with those of reference standards. The seven samples were found to contain at least one of the following compounds: sildenafil, vardenafil, hydroxyhomosildenafil, homosildenafil and acetildenafil. The five compounds were simultaneously determined by LC-ESI-MS/MS in multiple reactions monitoring (MRM) scan mode. The method has been validated for accuracy, precision, linearity and sensitivity. © 2005 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/116594
ISSN: 00219673
DOI: 10.1016/j.chroma.2005.11.103
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

WEB OF SCIENCETM
Citations

106
checked on May 23, 2018

Page view(s)

46
checked on May 25, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.