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https://scholarbank.nus.edu.sg/handle/10635/116194
| Title: | A high throughput enzyme-linked immunosorbent assay for inhibitors of the interaction between retinoblastoma protein and the Leu-X-Cys-X-Glu motif | Authors: | Ellsmore, V.A. Teoh, A.P. Ganesan, A. |
Issue Date: | 1997 | Citation: | Ellsmore, V.A.,Teoh, A.P.,Ganesan, A. (1997). A high throughput enzyme-linked immunosorbent assay for inhibitors of the interaction between retinoblastoma protein and the Leu-X-Cys-X-Glu motif. Journal of Biomolecular Screening 2 (4) : 207-211. ScholarBank@NUS Repository. | Abstract: | A 96-well enzyme-linked immunosorbent assay was developed to discover compounds that inhibit the binding of the Leu-X-Cys-X-Glu (LXCXE) motif to the retinoblastoma tumor suppressor protein (pRB). The assay uses a LXCXE-containing multiple antigenic peptide (MAP) which is immobilized on a microtiter plate. A truncated form of pRB is added and the amount bound detected by a monoclonal antibody. This rapid assay was employed in high throughput screening of crude natural product extracts and discrete compounds. © 1997 The Society for Biomolecular Screening, Inc. | Source Title: | Journal of Biomolecular Screening | URI: | http://scholarbank.nus.edu.sg/handle/10635/116194 | ISSN: | 10870571 |
| Appears in Collections: | Staff Publications |
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