Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/116194
Title: A high throughput enzyme-linked immunosorbent assay for inhibitors of the interaction between retinoblastoma protein and the Leu-X-Cys-X-Glu motif
Authors: Ellsmore, V.A.
Teoh, A.P. 
Ganesan, A. 
Issue Date: 1997
Source: Ellsmore, V.A.,Teoh, A.P.,Ganesan, A. (1997). A high throughput enzyme-linked immunosorbent assay for inhibitors of the interaction between retinoblastoma protein and the Leu-X-Cys-X-Glu motif. Journal of Biomolecular Screening 2 (4) : 207-211. ScholarBank@NUS Repository.
Abstract: A 96-well enzyme-linked immunosorbent assay was developed to discover compounds that inhibit the binding of the Leu-X-Cys-X-Glu (LXCXE) motif to the retinoblastoma tumor suppressor protein (pRB). The assay uses a LXCXE-containing multiple antigenic peptide (MAP) which is immobilized on a microtiter plate. A truncated form of pRB is added and the amount bound detected by a monoclonal antibody. This rapid assay was employed in high throughput screening of crude natural product extracts and discrete compounds. © 1997 The Society for Biomolecular Screening, Inc.
Source Title: Journal of Biomolecular Screening
URI: http://scholarbank.nus.edu.sg/handle/10635/116194
ISSN: 10870571
Appears in Collections:Staff Publications

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