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|Title:||A high throughput enzyme-linked immunosorbent assay for inhibitors of the interaction between retinoblastoma protein and the Leu-X-Cys-X-Glu motif|
|Citation:||Ellsmore, V.A.,Teoh, A.P.,Ganesan, A. (1997). A high throughput enzyme-linked immunosorbent assay for inhibitors of the interaction between retinoblastoma protein and the Leu-X-Cys-X-Glu motif. Journal of Biomolecular Screening 2 (4) : 207-211. ScholarBank@NUS Repository.|
|Abstract:||A 96-well enzyme-linked immunosorbent assay was developed to discover compounds that inhibit the binding of the Leu-X-Cys-X-Glu (LXCXE) motif to the retinoblastoma tumor suppressor protein (pRB). The assay uses a LXCXE-containing multiple antigenic peptide (MAP) which is immobilized on a microtiter plate. A truncated form of pRB is added and the amount bound detected by a monoclonal antibody. This rapid assay was employed in high throughput screening of crude natural product extracts and discrete compounds. © 1997 The Society for Biomolecular Screening, Inc.|
|Source Title:||Journal of Biomolecular Screening|
|Appears in Collections:||Staff Publications|
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