Please use this identifier to cite or link to this item:
Title: Increased expression of growth hormone and prolactin receptors in hepatocellular carcinomas
Authors: García-Caballero, T.
Mertani, H.M.
Lambert, A.
Gallego, R.
Fraga, M.
Pintos, E.
Forteza, J.
Chevallier, M.
Lobie, P.E. 
Vonderhaar, B.K.
Beiras, A.
Morel, G.
Keywords: Hepatocyte
In situ hybridization
mRNA expression
Issue Date: 2000
Source: García-Caballero, T.,Mertani, H.M.,Lambert, A.,Gallego, R.,Fraga, M.,Pintos, E.,Forteza, J.,Chevallier, M.,Lobie, P.E.,Vonderhaar, B.K.,Beiras, A.,Morel, G. (2000). Increased expression of growth hormone and prolactin receptors in hepatocellular carcinomas. Endocrine 12 (3) : 265-271. ScholarBank@NUS Repository.
Abstract: The liver is an essential target tissue for growth hormone (GH) and prolactin (PRL). The aim of this study was to determine the in situ expression of growth hormone receptor (GHR) and prolactin receptor (PRLR) in hepatocellular carcinomas and to compare the results with normal liver. For this purpose, in situ hybridization (ISH) and immunohistochemical techniques were performed and several tests were conducted to validate the results. By radioactive ISH, all the hepatocellular carcinomas studied showed labeling for GHR and PRLR mRNAs. Relative expression levels, determined by computer- assisted microdensity, were higher in hepatocellular carcinomas than in normal liver. Immunohistochemistry led us to confirm the constant expression of both receptor proteins in hepatocellular carcinomas and normal liver and to demonstrate their localization not only in the cytoplasm but also in the nucleus. These results confirm that the liver is a major GH and PRL target tissue and suggest that in hepatocellular carcinomas the proliferative effects of these hormones may be increased by a higher expression of their receptors.
Source Title: Endocrine
ISSN: 1355008X
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Mar 8, 2018

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.