High-throughput synchronization of mammalian cell cultures by spiral microfluidics

Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-62703-733-4_1

Title:	High-throughput synchronization of mammalian cell cultures by spiral microfluidics
Authors:	Lee, W.C. Bhagat, A.A.S. Lim, C.T.
Keywords:	Cancer cells Cell cycle synchronization Cell separation Microfluidics Stem cells
Issue Date:	2014
Citation:	Lee, W.C.,Bhagat, A.A.S.,Lim, C.T. (2014). High-throughput synchronization of mammalian cell cultures by spiral microfluidics. Methods in Molecular Biology 1104 : 3-13. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-62703-733-4_1
Abstract:	The development of mammalian cell cycle synchronization techniques has greatly advanced our understanding of many cellular regulatory events and mechanisms specific to different phases of the cell cycle. In this chapter, we describe a high-throughput microfluidic-based approach for cell cycle synchronization. By exploiting the relationship between cell size and its phase in the cell cycle, large numbers of synchronized cells can be obtained by size fractionation in a spiral microfluidic channel. Protocols for the synchronization of primary cells such as mesenchymal stem cells, and immortal cell lines such as Chinese hamster ovarian cells (CHO-CD36) and HeLa cells are provided as examples. © 2014 Springer Science+Business Media, LLC.
Source Title:	Methods in Molecular Biology
URI:	http://scholarbank.nus.edu.sg/handle/10635/114511
ISBN:	9781627037327
ISSN:	10643745
DOI:	10.1007/978-1-62703-733-4_1
Appears in Collections:	Staff Publications

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