Please use this identifier to cite or link to this item:
Title: Microgravimetric DNA sensor based on quartz crystal microbalance: Comparison of oligonucleotide immobilization methods and the application in genetic diagnosis
Authors: Zhou, X.C.
Huang, L.Q. 
Li, S.F.Y. 
Keywords: β-thalassaemia
DNA hybridization
Quartz crystal microbalance
Issue Date: 2001
Citation: Zhou, X.C., Huang, L.Q., Li, S.F.Y. (2001). Microgravimetric DNA sensor based on quartz crystal microbalance: Comparison of oligonucleotide immobilization methods and the application in genetic diagnosis. Biosensors and Bioelectronics 16 (1-2) : 85-95. ScholarBank@NUS Repository.
Abstract: We report on the study of immobilization DNA probes onto quartz crystal oscillators by self-assembly technique to form variety types of mono- and multi-layered sensing films towards the realization of DNA diagnostic devices. A 18-mer DNA probe complementary to the site of genetic β-thalassaemia mutations was immobilized on the electrodes of QCM by covalent bonding or electrostatic adsorption on polyelectrolyte films to form mono- or multi-layered sensing films by self-assembled process. Hybridization was induced by exposure of the QCMs immobilized with DNA probe to a test solution containing the target nucleic acid sequences. The kinetics of DNA probe immobilization and hybridization with the fabricated DNA sensors were studied via in-situ frequency changes. The characteristics of QCM sensors containing mono- or multi-layered DNA probe constructed by direct chemical bonding, avidin-biotin interaction or electrostatic adsorption on polyelectrolyte films were compared. Results indicated that the DNA sensing films fabricated by immobilization of biotinylated DNA probe to avidin provide fast sensor response and high hybridization efficiencies. The effects of ionic strength of the buffer solution and the concentration of target nucleic acid used in hybridization were also studied. The fabricated DNA biosensor was used to detect a set of real samples. We conclude that the microgravimetric DNA sensor with its direct detection of amplified products provide a rapid, low cost and convenient diagnostic method for genetic disease. © 2001 Elsevier Science B.V.
Source Title: Biosensors and Bioelectronics
ISSN: 09565663
DOI: 10.1016/S0956-5663(00)00136-6
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jun 19, 2018


checked on Jun 11, 2018

Page view(s)

checked on May 4, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.