Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cub.2004.09.048
Title: Drosophila T box proteins break the symmetry of hedgehog-dependent activation of wingless
Authors: Buescher, M.
Svendsen, P.C.
Tio, M.
Miskolczi-McCallum, C.
Tear, G.
Brook, W.J.
Chia, W. 
Issue Date: 5-Oct-2004
Citation: Buescher, M., Svendsen, P.C., Tio, M., Miskolczi-McCallum, C., Tear, G., Brook, W.J., Chia, W. (2004-10-05). Drosophila T box proteins break the symmetry of hedgehog-dependent activation of wingless. Current Biology 14 (19) : 1694-1702. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cub.2004.09.048
Abstract: Background: Segmentation of the Drosophila embryo is a classic paradigm for pattern formation during development. The Wnt-1 homolog Wingless (Wg) is a key player in the establishment of a segmentally reiterated pattern of cell type specification. The intrasegmental polarity of this pattern depends on the precise positioning of the Wg signaling source anterior to the Engrailed (En)/Hedgehog (Hh) domain. Proper polarity of epidermal segments requires an asymmetric response to the bidirectional Hh signal: wg is activated in cells anterior to the Hh signaling source and is restricted from cells posterior to this signaling source. Results: Here we report that Midline (Mid) and H15, two highly related T box proteins representing the orthologs of zebrafish hrT and mouse Tbx20, are novel negative regulators of wg transcription and act to break the symmetry of Hh signaling. Loss of mid and H15 results in the symmetric outcome of Hh signaling: the establishment of wg domains anterior and posterior to the signaling source predominantly, but not exclusively, in odd-numbered segments. Accordingly, loss of mid and H15 produces defects that mimic a wg gain-of-function phenotype. Misexpression of mid represses wg and produces a weak/moderate wg loss-of-function phenocopy. Furthermore, we show that loss of mid and H15 results in an anterior expansion of the expression of serrate (ser) in every segment, representing a second instance of target gene repression downstream of Hh signaling in the establishment of segment polarity. Conclusions: The data we present here indicate that mid and H15 are important components in pattern formation in the ventral epidermis. In odd-numbered abdominal segments, Mid/H15 activity plays an important role in restricting the expression of Wg to a single domain.
Source Title: Current Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/113448
ISSN: 09609822
DOI: 10.1016/j.cub.2004.09.048
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

31
checked on Jun 14, 2018

WEB OF SCIENCETM
Citations

31
checked on May 7, 2018

Page view(s)

37
checked on May 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.