Please use this identifier to cite or link to this item:
|Title:||Novel actin-like filament structure from Clostridium tetani|
|Citation:||Popp, D., Narita, A., Lee, L.J., Ghoshdastider, U., Xue, B., Srinivasan, R., Balasubramanian, M.K., Tanaka, T., Robinson, R.C. (2012-06-15). Novel actin-like filament structure from Clostridium tetani. Journal of Biological Chemistry 287 (25) : 21121-21129. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M112.341016|
|Abstract:||Eukaryotic F-actin is constructed from two protofilaments that gently wind around each other to form a helical polymer. Several bacterial actin-like proteins (Alps) are also known to form F-actin-like helical arrangements from two protofilaments, yet with varied helical geometries. Here, we report a unique filament architecture of Alp12 from Clostridium tetani that is constructed from four protofilaments. Through fitting of an Alp12 monomer homology model into the electron microscopy data, the filament was determined to be constructed from two antiparallel strands, each composed of two parallel protofilaments. These four protofilaments form an open helical cylinder separated by a wide cleft. The molecular interactions within single protofilaments are similar to F-actin, yet interactions between protofilaments differ from those in F-actin. The filament structure and assembly and disassembly kinetics suggest Alp12 to be a dynamically unstable force-generating motor involved in segregating the pE88 plasmid, which encodes the lethal tetanus toxin, and thus a potential target for drug design. Alp12 can be repeatedly cycled between states of polymerization and dissociation, making it a novel candidate for incorporation into fuel-propelled nanobiopolymer machines. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.|
|Source Title:||Journal of Biological Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 22, 2018
WEB OF SCIENCETM
checked on May 7, 2018
checked on Feb 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.