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|Title:||Use of capillary electrophoresis for thalassaemia screening|
|Authors:||Law, H.-Y. |
|Source:||Law, H.-Y., Ng, I., Ong, J., Ong, C.-N. (1994). Use of capillary electrophoresis for thalassaemia screening. Biomedical Chromatography 8 (4) : 175-179. ScholarBank@NUS Repository. https://doi.org/10.1002/bmc.1130080406|
|Abstract:||This paper describes the use of high-performance capillary electrophoresis (HPCE) for screening of thalassaemias. Preparation of globin was performed by adding 1 mL of haemolysate to 20 volumes of cold acidified acetone. Separation of globins was accomplished in a 25 mM disodium phosphate buffer at pH 11.8 using an uncoated capillary column of 50 cm x 75 μm (i.d.). Distinct peaks of alpha, beta and gamma were resolved within 6 min. The coefficient of variations for within-day and between-day runs were 4.7% and 6.37%, respectively. Using this simple and rapid procedure up to 30 specimens could be analysed in a single day. This method appears to be reliable and can be used for mass screening of various haemoglobinopathies.|
|Source Title:||Biomedical Chromatography|
|Appears in Collections:||Staff Publications|
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