Please use this identifier to cite or link to this item: https://doi.org/10.1007/s002510050422
Title: The β7 integrin gene (Itgb-7) promoter is responsive to TGF-β1: Defining control regions
Authors: Lim, S.P. 
Leung, E.
Krissansen, G.W.
Keywords: β7 integrin gene
Phosphorylation
Promoter elements
TGFβ1
Issue Date: 1998
Citation: Lim, S.P., Leung, E., Krissansen, G.W. (1998). The β7 integrin gene (Itgb-7) promoter is responsive to TGF-β1: Defining control regions. Immunogenetics 48 (3) : 184-195. ScholarBank@NUS Repository. https://doi.org/10.1007/s002510050422
Abstract: The β7 integrins LPAM-1 (α4β7) and M290 (αEβ7) mediate the homing of lymphocytes to gut-associated lymphoid tissue, and the proposed retention of intraepithelial lymphocytes (IEL), respectively. Here we show that the gut mucosal cytokine TGF- β1 increases the expression of β7 and αE subunit mRNA transcripts and the cell-surface expression of M290 on T cells, and that it decreases the level of α4 integrin transcripts. Induced β7 integrin gene expression was inhibited by the protein tyrosine kinase inhibitor genistein, implicating a role for tyrosine phosphorylation. An analysis of the P7 integrin gene promoter revealed three DNAse I hypersensitivity sites, two of which mapped to the 5' and 3' ends of a promoter fragment (nucleotides + 690 to +63) that directed both the basal and the TGF-β1-induced expression of a heterologous reporter gene. Deletion analysis identified two TGF-β1 response regions encompassing nucleotides -509 to -398 (TGFBRR1), and -122 to +32 (TGFBRR2). TGFBRR1 interacted with at least five protein complexes, whose binding could be induced with TGF-β1 stimulation and could be antagonized by TGFBRR2 which harbored both similar and distinctive cis-elements. TGFBRR2 interacted specifically with at least two major nuclear protein complexes, whose binding was phosphorylation dependent. These data provide new insights into the mechanism by which TGF-β may switch LPAM-1(+ ve) migrating T cells to express M290, facilitating their retention in the gut.
Source Title: Immunogenetics
URI: http://scholarbank.nus.edu.sg/handle/10635/112137
ISSN: 00937711
DOI: 10.1007/s002510050422
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

44
checked on Dec 12, 2018

WEB OF SCIENCETM
Citations

41
checked on Dec 12, 2018

Page view(s)

18
checked on Nov 16, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.