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Title: The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment
Authors: Klumperman, J.
Schweizer, A.
Clausen, H.
Tang, B.L. 
Hong, W. 
Oorschot, V.
Hauri, H.-P.
Keywords: β-COP
ER-Golgi intermediate compartment
Immunoelectron microscopy
Protein recycling
Issue Date: Nov-1998
Source: Klumperman, J.,Schweizer, A.,Clausen, H.,Tang, B.L.,Hong, W.,Oorschot, V.,Hauri, H.-P. (1998-11). The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment. Journal of Cell Science 111 (22) : 3411-3425. ScholarBank@NUS Repository.
Abstract: To establish recycling routes in the early secretory pathway we have studied the recycling of the ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 in HepG2 cells. Immunofluorescence microscopy showed progressive concentration of ERGIC-53 in the Golgi area at 15°C, Upon rewarming to 37°C ERGIC-53 redistributed into the cell periphery often via tubular processes that largely excluded anterograde transported albumin. Immunogold labeling of cells cultured at 37°C revealed ERGIC-53 predominantly in characteristic β-COP-positive tubulo-vesicular clusters both near the Golgi apparatus and in the cell periphery. Concentration of ERGIC-53 at 15°C resulted from both accumulation of ERGIC-53 in the ERGIC and movement of ERGIC membranes closer to the Golgi apparatus. Upon rewarming to 37°C the labeling of ERGIC-53 in the ERGIC rapidly returned to normal levels whereas ERGIC-53's labeling in the cis-Golgi was unchanged. Temperature manipulations had no effect on the average number of ERGIC-53 clusters. Density gradient centrifugation indicated that the surplus ERGIC-53 accumulating in the ERGIC at 15°C was rapidly transported to the ER upon rewarming. These results suggest that the ERGIC is a dynamic membrane system composed of a constant average number of clusters and that the major recycling pathway of ERGIC-53 bypasses the Golgi apparatus.
Source Title: Journal of Cell Science
ISSN: 00219533
Appears in Collections:Staff Publications

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