Please use this identifier to cite or link to this item: https://doi.org/10.1007/s002990050099
Title: Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro
Authors: Pua, E.-C. 
Sim, G.-E. 
Chi, G.-L. 
Kong, L.-F.
Keywords: AgNO3
aminoethoxyvinylglycine
Chinese radish
ethylene
putrescine
Raphanus sativus var. longipinnatus
shoot regeneration
Issue Date: 1996
Citation: Pua, E.-C.,Sim, G.-E.,Chi, G.-L.,Kong, L.-F. (1996). Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro. Plant Cell Reports 15 (9) : 685-690. ScholarBank@NUS Repository. https://doi.org/10.1007/s002990050099
Abstract: The role of ethylene and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey cv. Red Coat) was investigated. Explants were recalcitrant in culture, but exogenous application of ethylene inhibitor 120-30 μM aminoethoxyvinylglycine (AVG) or AgNO3] enhanced shoot regeneration of explants grown on medium supplemented with 2 mg/l N6-benzyladenine and 1 mg/l 1-naphthaleneacetic acid. The best regeneration occurred in the medium containing AgNO3 in combination with AVG. Culture medium solidified with agarose in the presence of AgNO3 but not AVG was also beneficial to shoot regeneration. Exogenous putrescine, 2-chloroethylphosphonic acid and 1- aminocyclopropane 1-carboxylate had no effect on shoot regeneration. However, regeneration was greatly promoted by 10-25 mM putrescine in combination with 30 μM AgNO3 or AVG. Explants with high regenerability grown in the presence of AgNO3 or in combination with putrescine emanated high levels of ethylene throughout the 21-d culture period. By contrast, AVG or putrescine alone resulted in a decrease in ethylene production. For rooting of shoot cuttings, IAA and IBA at 1-5 mg/l were more effective than NAA.
Source Title: Plant Cell Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/112097
ISSN: 07217714
DOI: 10.1007/s002990050099
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