Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/112087
Title: Steroid metabolism in teleost gonads: purification and identification of metabolites by high-performance liquid chromatography
Authors: Venkatesh, B. 
Cheong Huat Tan 
Kime, D.E.
Toong Jin Lam 
Keywords: androgens
corticosteroids
estrogen
gonadal steroids
HPLC
progestins
steroids
teleosts
Issue Date: Jun-1992
Source: Venkatesh, B.,Cheong Huat Tan,Kime, D.E.,Toong Jin Lam (1992-06). Steroid metabolism in teleost gonads: purification and identification of metabolites by high-performance liquid chromatography. Steroids 57 (6) : 276-281. ScholarBank@NUS Repository.
Abstract: A simple, efficient, and comprehensive technique for the purification, identification, and quantitation of the common steroid metabolites synthesized by the gonads of teleosts involving five systems of highperformance liquid chromatography (HPLC) was developed. Steroid standards were identified in HPLC by UV absorption at 254 nm or280 nm, by differential refractive index, or by using radioactive standards. Metabolites that do not absorb UV light and are not resolved in the isocratic HPLC systems were identified in thin-layer chromatography following purification by HPLC. By using this technique, most of the steroid metabolites, including some polar metabolites, synthesized by the gonadal tissues of the teleosts can be purified within three steps of chromatography. The HPLC systems reported here are also useful in identifying the chromium trioxide oxidized products of metabolites, such as triols andtetrols, which considerably narrows down the number of probable metabolites. (Steroids 57:276-281, 1992). © 1992.
Source Title: Steroids
URI: http://scholarbank.nus.edu.sg/handle/10635/112087
ISSN: 0039128X
Appears in Collections:Staff Publications

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