Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1020653028685
Title: Role of phosphate and kinetic characteristics of complete iron release from native pig spleen ferritin-Fe
Authors: Huang, H.-Q.
Lin, Q.-M.
Kong, B.
Zeng, R.-Y.
Qiao, Y.-H.
Chen, C.-H. 
Zhang, F.-Z.
Xu, L.-S.
Keywords: Ascorbic acid and dithionite
Kinetics and order
Phosphate role and channel function
Pig spleen ferritin
Issue Date: 1999
Citation: Huang, H.-Q., Lin, Q.-M., Kong, B., Zeng, R.-Y., Qiao, Y.-H., Chen, C.-H., Zhang, F.-Z., Xu, L.-S. (1999). Role of phosphate and kinetic characteristics of complete iron release from native pig spleen ferritin-Fe. Journal of Protein Chemistry 18 (4) : 497-504. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1020653028685
Abstract: The kinetics for complete iron release showing biphasic behavior from pig spleen ferritin-Fe (PSFF) was measured by spectrophotometry. The native core within the PSFF shell consisted of 1682 hydroxide Fe3+ and 13 phosphate molecules. Inhibition kinetics for complete iron release was measure by differential spectrophotometry in the presence of phosphate; the process was clearly divided into two phases involving a first-order reaction at an increasing rate of 46.5 Fe3+/PSFF/min on the surface of the iron core and a zero-order reaction at a decreasing rate of 6.67 Fe3+/PSFF/min inside the core. The kinetic equation [C(PSFF-Fe3+)(max) - C(PSFF-Fe3+((t)](1/2) = T(max) -T(t) gives the transition time between the two rates and represents the complex kinetic characteristics. The rate was directly accelerated twofold by a mixed reducer of dithionite and ascorbic acid. These results suggest that the channel of the PSFF shell may carry out multiple functions for iron metabolism and storage and that the phosphate strongly affects the rate of iron release.
Source Title: Journal of Protein Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/112059
ISSN: 02778033
DOI: 10.1023/A:1020653028685
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