Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/111783
Title: Analysis of O6-methylguanine-DNA methyltransferase in individual human cells by quantitative immunofluorescence microscopy
Authors: Belanich, M.
Ayi, T.-C. 
Li, B.F.L. 
Kibitel, J.T.
Grob, D.W.
Randall, T.
White, A.B.
Citron, M.L.
Yarosh, D.B.
Issue Date: 1994
Citation: Belanich, M.,Ayi, T.-C.,Li, B.F.L.,Kibitel, J.T.,Grob, D.W.,Randall, T.,White, A.B.,Citron, M.L.,Yarosh, D.B. (1994). Analysis of O6-methylguanine-DNA methyltransferase in individual human cells by quantitative immunofluorescence microscopy. Oncology Research 6 (3) : 129-137. ScholarBank@NUS Repository.
Abstract: A quantitative assay of immunofluorescence is described that can be performed on individual cells from standard pathologic specimens using fluorescence microscopy. The technique has been applied to measurement of O6-methylguanine-DNA methyltransferase, a DNA repair protein that is a molecular marker for resistance to chloroethylnitrosoureas used in cancer chemotherapy. The immunofluorescence assay makes use of monoclonal antibodies with specificity for human transferase, fluorescence microscopy with digital imaging, fluorescent bead internal standards, and computerized image analysis. This method is specific for the transferase, produces results correlated with activity measurements, and yields new data about tissue heterogeneity and subcellular localization previously unavailable with standard assay methods. © 1994.
Source Title: Oncology Research
URI: http://scholarbank.nus.edu.sg/handle/10635/111783
ISSN: 09650407
Appears in Collections:Staff Publications

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