Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/111737
Title: Spliced leader-associated RNA of trypanosomes: Sequence conservation and association with protein components common to trans-spliceosomal ribonucleoproteins
Authors: Palfi, Z.
Xu, G.-L. 
Bindereif, A.
Issue Date: 2-Dec-1994
Citation: Palfi, Z.,Xu, G.-L.,Bindereif, A. (1994-12-02). Spliced leader-associated RNA of trypanosomes: Sequence conservation and association with protein components common to trans-spliceosomal ribonucleoproteins. Journal of Biological Chemistry 269 (48) : 30620-30625. ScholarBank@NUS Repository.
Abstract: A spliced leader-associated (SLA) RNA recently identified in Trypanosoma brucei has been proposed as a U5 RNA analog in trans-splicing, based on in vivo psoralen cross-linking to the spliced leader (SL) RNA and a short stretch of homology to a conserved U5 RNA sequence. Here we show that the SLA RNA is in the form of an RNP containing common proteins that also occur in the known trans-spliceosomal snRNPs of T. brucei, the SL, U2, and U4/U6 snRNPs. Therefore the SLA RNP is a member of a larger family of related RNPs. The immunoprecipitation of the SLA RNA by antibodies against common proteins does not require an association between the SLA RNA and the SL RNP. To identify sequences of potential functional importance and to establish a secondary structure model, we have sequenced the SLA RNA and cloned its gene from a related trypanosomatid, Leptomonas seymouri. A sequence comparison between the T. brucei and L. seymouri SLA RNAs reveals that the U5-homologous sequence element is not conserved.
Source Title: Journal of Biological Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/111737
ISSN: 00219258
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

34
checked on Nov 9, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.