Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1003795
Title: The Condition-Dependent Transcriptional Landscape of Burkholderia pseudomallei
Authors: Ooi, W.F.
Ong, C.
Nandi, T.
Kreisberg, J.F.
Chua, H.H.
Sun, G.
Chen, Y.
Mueller, C.
Conejero, L.
Eshaghi, M.
Ang, R.M.L.
Liu, J.
Sobral, B.W.
Korbsrisate, S.
Gan, Y.H.
Titball, R.W.
Bancroft, G.J.
Valade, E.
Tan, P. 
Issue Date: Sep-2013
Source: Ooi, W.F., Ong, C., Nandi, T., Kreisberg, J.F., Chua, H.H., Sun, G., Chen, Y., Mueller, C., Conejero, L., Eshaghi, M., Ang, R.M.L., Liu, J., Sobral, B.W., Korbsrisate, S., Gan, Y.H., Titball, R.W., Bancroft, G.J., Valade, E., Tan, P. (2013-09). The Condition-Dependent Transcriptional Landscape of Burkholderia pseudomallei. PLoS Genetics 9 (9) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1003795
Abstract: Burkholderia pseudomallei (Bp), the causative agent of the often-deadly infectious disease melioidosis, contains one of the largest prokaryotic genomes sequenced to date, at 7.2 Mb with two large circular chromosomes (1 and 2). To comprehensively delineate the Bp transcriptome, we integrated whole-genome tiling array expression data of Bp exposed to >80 diverse physical, chemical, and biological conditions. Our results provide direct experimental support for the strand-specific expression of 5,467 Sanger protein-coding genes, 1,041 operons, and 766 non-coding RNAs. A large proportion of these transcripts displayed condition-dependent expression, consistent with them playing functional roles. The two Bp chromosomes exhibited dramatically different transcriptional landscapes - Chr 1 genes were highly and constitutively expressed, while Chr 2 genes exhibited mosaic expression where distinct subsets were expressed in a strongly condition-dependent manner. We identified dozens of cis-regulatory motifs associated with specific condition-dependent expression programs, and used the condition compendium to elucidate key biological processes associated with two complex pathogen phenotypes - quorum sensing and in vivo infection. Our results demonstrate the utility of a Bp condition-compendium as a community resource for biological discovery. Moreover, the observation that significant portions of the Bp virulence machinery can be activated by specific in vitro cues provides insights into Bp's capacity as an "accidental pathogen", where genetic pathways used by the bacterium to survive in environmental niches may have also facilitated its ability to colonize human hosts. © 2013 Ooi et al.
Source Title: PLoS Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/110639
ISSN: 15537390
DOI: 10.1371/journal.pgen.1003795
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