Please use this identifier to cite or link to this item: https://doi.org/10.1002/rcm.2878
Title: Qualitative and quantitative analysis of toosendanin in Melia toosendan Sieb. et Zucc (Meliaceae) with liquid chromatography/tandem mass spectrometry
Authors: Eng, S.O.
Choon, N.O. 
Issue Date: 2007
Citation: Eng, S.O., Choon, N.O. (2007). Qualitative and quantitative analysis of toosendanin in Melia toosendan Sieb. et Zucc (Meliaceae) with liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry 21 (4) : 589-598. ScholarBank@NUS Repository. https://doi.org/10.1002/rcm.2878
Abstract: Toosendanin (TSN) is a triterpenoid derivative found in Melia toosendan Sieb. Et Zucc (Meliaceae) or chinaberry. TSN present in the medicinal plants was first isolated and established by spectroscopic methods. In this report, high-performance liquid chromatography (HPLC) separation using columns of smaller particle size with tandem mass spectrometry (MSn) was used for the rapid determination of TSN in botanical extracts. A comparison of different fragmentation patterns shows that the results from positive and negative ion electrospray ionization (ESI)-MSn are complementary. The two modes can yield structurally significant information for the characterization and rapid identification of TSN in botanical extracts. The data obtained showed that MS3 generated more characteristic ions that are useful for the identification of TSN in unknown samples. The separation of TSN was achieved with a water/acetonitrile gradient system using a short C18 reversed-phase column with small particle size (50 X 2.0 mm, 3.5 μm). With LC/MS, the quantitative analysis of TSN in the botanical extracts was done using external standard calibration and the method precision was found to vary from 4.3 to 7.6% (RSD, n = 5) on different days. Copyright © 2007 John Wiley & Sons, Ltd.
Source Title: Rapid Communications in Mass Spectrometry
URI: http://scholarbank.nus.edu.sg/handle/10635/109562
ISSN: 09514198
DOI: 10.1002/rcm.2878
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