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|Title:||Label-free quantitative proteomics reveals differentially regulated proteins in the latex of sticky diseased Carica papaya L. plants|
Label-free quantitative proteomics
|Source:||Rodrigues, S.P., Ventura, J.A., Aguilar, C., Nakayasu, E.S., Choi, H., Sobreira, T.J.P., Nohara, L.L., Wermelinger, L.S., Almeida, I.C., Zingali, R.B., Fernandes, P.M.B. (2012-06-18). Label-free quantitative proteomics reveals differentially regulated proteins in the latex of sticky diseased Carica papaya L. plants. Journal of Proteomics 75 (11) : 3191-3198. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jprot.2012.03.021|
|Abstract:||Papaya meleira virus (PMeV) is so far the only described laticifer-infecting virus, the causal agent of papaya (Carica papaya L.) sticky disease. The effects of PMeV on the laticifers' regulatory network were addressed here through the proteomic analysis of papaya latex. Using both 1-DE- and 1D-LC-ESI-MS/MS, 160 unique papaya latex proteins were identified, representing 122 new proteins in the latex of this plant. Quantitative analysis by normalized spectral counting revealed 10 down-regulated proteins in the latex of diseased plants, 9 cysteine proteases (chymopapain) and 1 latex serine proteinase inhibitor. A repression of papaya latex proteolytic activity during PMeV infection was hypothesized. This was further confirmed by enzymatic assays that showed a reduction of cysteine-protease-associated proteolytic activity in the diseased papaya latex. These findings are discussed in the context of plant responses against pathogens and may greatly contribute to understand the roles of laticifers in plant stress responses. © 2012 Elsevier B.V.|
|Source Title:||Journal of Proteomics|
|Appears in Collections:||Staff Publications|
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