Please use this identifier to cite or link to this item: https://doi.org/10.1038/sj.bjp.0706014
Title: Role of hydrogen sulphide in haemorrhagic shock in the rat: Protective effect of inhibitors of hydrogen sulphide biosynthesis
Authors: Mok, Y.-Y.P.
Mohammed Atan, M.S.B. 
Ping, C.Y.
Jing, W.Z.
Bhatia, M. 
Moochhala, S. 
Moore, P.K. 
Keywords: β-cyanoalanine
DL-propargylglycine
Glibenclamide
Haemorrhagic shock
Hydrogen sulphide
Issue Date: Dec-2004
Citation: Mok, Y.-Y.P., Mohammed Atan, M.S.B., Ping, C.Y., Jing, W.Z., Bhatia, M., Moochhala, S., Moore, P.K. (2004-12). Role of hydrogen sulphide in haemorrhagic shock in the rat: Protective effect of inhibitors of hydrogen sulphide biosynthesis. British Journal of Pharmacology 143 (7) : 881-889. ScholarBank@NUS Repository. https://doi.org/10.1038/sj.bjp.0706014
Abstract: 1 Haemorrhagic shock (60 min) in the anaesthetized rat resulted in a prolonged fall in the mean arterial blood pressure (MAP) and heart rate (HR). 2 Pre-treatment (30 min before shock) or post-treatment (60 min after shock) with inhibitors of cystathionine γ lyase (CSE; converts cysteine into hydrogen sulphide (H 2S)), d1-propargylglycine or β-cyanoalanine (50 mg kg -1, i.V.), or glibenclamide (40 mg kg -1, i.p.), produced a rapid, partial restoration in MAP and HR. Neither saline nor DMSO affected MAP or HR. 3 Plasma H 2S concentration was elevated 60min after blood withdrawal (37.5 ± 1.3 μm, n = 18 c.f. 28.9 ± 1.4 μm, n = 15, P < 0.05). 4 The conversion of cysteine to H 2S by liver (but not kidney) homogenates prepared from animals killed 60 min after withdrawal of blood was significantly increased (52.1 ± 1.6 c.f. 39.8 ± 4.1 nmol mg protein -1, n = 8, P < 0.05) as was liver CSE mRNA (2.7 ×). Both PAG (IC 50, 55.0 ± 3.2 μm) and BCA (IC 50, 6.5 ± 1.2 μm) inhibited liver H 2S synthesizing activity in vitro. 5 Pre-treatment of animals with PAG or BCA (50 mg kg -1, i.p.) but not glibenclamide (40 mg kg -1, i.p., K ATP channel inhibitor) abolished the rise in plasma H 2S in animals exposed to 60 min haemorrhagic shock and prevented the augmented biosynthesis of H 2S from cysteine in liver. 6 These results demonstrate that H 2S plays a role in haemorrhagic shock in the rat. CSE inhibitors may provide a novel approach to the treatment of haemorrhagic shock.
Source Title: British Journal of Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/108059
ISSN: 00071188
DOI: 10.1038/sj.bjp.0706014
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

176
checked on Sep 24, 2018

WEB OF SCIENCETM
Citations

164
checked on Sep 24, 2018

Page view(s)

39
checked on Sep 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.